WatershedCounting3D Instructions 1. Install ImageJ 2. Download WatershedCounting_3D.jar file 3. IMPORTANT: Windows may rename this file WatershedCounting_3D.zip or WatershedCounting_3D.jar.zip If this happens, change the name back to WatershedCounting_3D.jar 4. Copy WatershedCounting_3D.jar to the ImageJ/PlugIns Folder 5. Restart ImageJ 6. Under the plug-ins menu, locate the WatershedCounting menu item. 7. Load an RGB z-stack into ImageJ, then press the WatershedCounting menu item. Please email Questions and comments to: thomas.gniadek@yale.edu